Research Article

Erratic Male Meiosis Resulting in 2𝑛 Pollen Grain Formation in a 4x Cytotype (2𝑛=28) of Ranunculus laetus Wall. ex Royle

Figure 4

(a–i) Meiocytes with erratic male meiosis at second meiotic division in R. laetus. (a) Unequal distribution of chromosomes (10 : 18) at M-II. (b) Scattered chromosomes at anaphase-II (arrowed). (c) Random and unoriented distribution of chromosomes at M-II. (d) A PMC showing lagging of chromosomes (arrowed). (e) Tetrad with micronuclei (arrowed). (f) Out of the two PMCs, one showing restitution (unreduced) nuclei (arrowed) and the other with four haploid nuclei at T-II. (g) Sporads in group; dyads with micronuclei (arrowhead), dyads without micronuclei (blue arrows), and triads (red arrow). (h) Apparently stained fertile and unstained or lightly stained, and shriveled sterile (arrowed) pollen grains. (i) Apparently stained fertile 𝑛 (reduced, arrowhead) and 2 𝑛 (unreduced, arrowed) pollen grains. Scale bar = 10 μm (a–d); 20 μm (h).
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