Potential Canonical Wnt Pathway Activation in High-Grade Astrocytomas
(a) Kaplan-Meier plot illustrating the correlation of patient outcome with β-catenin-P654 expression levels in the subgroup of low-grade astrocytomas. Expression of β-catenin-P654 is directly associated with a prolonged overall survival (OS). P value represents multivariate associations of staining frequencies with OS. (b) Immunofluorescence staining showing colocalization of the stem cell-associated filament protein nestin (red) and β-catenin (green). DAPI (blue) was used for nuclear counterstaining. Representative staining is shown in a primary glioblastoma tissue (white arrow heads represent double-positive cells; scale bars represent 150 μm). (c) Graphs show β-catenin and nestin mRNA expression in NCH421k cells infected with scrambled control or a shRNA directed against β-catenin. Data are shown as mean values ± SEM of four technical replicates.
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