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The Scientific World Journal
Volume 2012, Article ID 716374, 5 pages
http://dx.doi.org/10.1100/2012/716374
Research Article

Immobilization of Peroxidase onto Magnetite Modified Polyaniline

1Laboratório de Química de Proteínas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Codigo Postal 131, 74001-970 Goiânia, GO, Brazil
2Grupo de Química de Carboydratos y Biotecnología de Alimentos, Departamento de Química Orgánica, Universidad de Murcia, Campus Espinardo, 34100 Murcia, Spain
3Unidade Universitária de Ciências Exatas e Tecnológicas, Campus Dr. Henrique Santillo, Universidade Estadual de Goiás, Rodovia BR 153 Km 98, Codigo Postal 459, 75132-903 Anápolis, GO, Brazil

Received 1 November 2011; Accepted 29 November 2011

Academic Editors: P. Andrade and C. Mateo

Copyright © 2012 Eduardo Fernandes Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Wang, S. Li, and J. Kan, “A hydrogen peroxide biosensor based on polyaniline/FTO,” Sensors and Actuators, B, vol. 137, no. 2, pp. 662–668, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. Q. Xu, J. J. Zhu, and X. Y. Hu, “Ordered mesoporous polyaniline film as a new matrix for enzyme immobilization and biosensor construction,” Analytica Chimica Acta, vol. 597, no. 1, pp. 151–156, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. X. Luo, A. J. Killard, A. Morrin, and M. R. Smyth, “Enhancement of a conducting polymer-based biosensor using carbon nanotube-doped polyaniline,” Analytica Chimica Acta, vol. 575, no. 1, pp. 39–44, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. N. G. R. Mathebe, A. Morrin, and E. I. Iwuoha, “Electrochemistry and scanning electron microscopy of polyaniline/ peroxidase-based biosensor,” Talanta, vol. 64, no. 1, pp. 115–120, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. C. C. Chen and Y. Gu, “Enhancing the sensitivity and stability of HRP/PANI/Pt electrode by implanted bovine serum albumin,” Biosensors and Bioelectronics, vol. 23, no. 6, pp. 765–770, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. Y. Yang and S. Mu, “Bioelectrochemical responses of the polyaniline horseradish peroxidase electrodes,” Journal of Electroanalytical Chemistry, vol. 432, no. 1-2, pp. 71–78, 1997. View at Google Scholar · View at Scopus
  7. K. F. Fernandes, C. S. Lima, H. Pinho, and C. H. Collins, “Immobilization of horseradish peroxidase onto polyaniline polymers,” Process Biochemistry, vol. 38, no. 9, pp. 1379–1384, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. K. F. Fernandes, C. S. Lima, F. M. Lopes, and C. H. Collins, “Properties of horseradish peroxidase immobilised onto polyaniline,” Process Biochemistry, vol. 39, no. 8, pp. 957–962, 2004. View at Publisher · View at Google Scholar · View at Scopus
  9. R. N. Silva, E. R. Asquieri, and K. F. Fernandes, “Immobilization of Aspergillus niger glucoamylase onto a polyaniline polymer,” Process Biochemistry, vol. 40, no. 3-4, pp. 1155–1159, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. L. L. A. Purcena, S. S. Caramori, S. Mitidieri, and K. F. Fernandes, “The immobilization of trypsin onto polyaniline for protein digestion,” Materials Science and Engineering C, vol. 29, no. 4, pp. 1077–1081, 2009. View at Publisher · View at Google Scholar · View at Scopus
  11. Z. M. Tahir, E. C. Alocilja, and D. L. Grooms, “Polyaniline synthesis and its biosensor application,” Biosensors and Bioelectronics, vol. 20, no. 8, pp. 1690–1695, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Laska, J. Włodarczyk, and W. Zaborska, “Polyaniline as a support for urease immobilization,” Journal of Molecular Catalysis—B, vol. 6, no. 6, pp. 549–553, 1999. View at Publisher · View at Google Scholar · View at Scopus
  13. X. Pan, S. Zhou, C. Chen, and J. Kan, “Preparation and properties of an uricase biosensor based on copolymer of o-aminophenol-aniline,” Sensors and Actuators, B, vol. 113, no. 1, pp. 329–334, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. J. M. Andrade, R. C. D. Peres, E. G. Oestreicher, O. A. C. Antunes, and C. Dariva, “Immobilization of d-hydantoinase in polyaniline,” Journal of Molecular Catalysis B, vol. 55, no. 3-4, pp. 185–188, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. K. F. Fernandes, C. S. Lima, F. M. Lopes, and C. H. Collins, “Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer,” Process Biochemistry, vol. 40, no. 11, pp. 3441–3445, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. S. S. Caramori and K. F. Fernandes, “The use of poly(ethylene terephthalate)-poly(aniline) composite for trypsin immobilisation,” Materials Science and Engineering C, vol. 28, no. 7, pp. 1159–1163, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. S. S. Caramori, F. N. De Faria, M. P. Viana, K. F. Fernandes, and L. B. Carvalho, “Trypsin immobilization on discs of polyvinyl alcohol glutaraldehyde/ polyaniline composite,” Materials Science and Engineering C, vol. 31, no. 2, pp. 252–257, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. D. F. M. Neri, V. M. Balcão, and R. S. Costa, “β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: a comparative experimental and modeling study of the galactooligosaccharides production,” in Proceedings of the 10th International Chemical and Biological Engineering Conference (CHEMPOR '08), E. C. Ferreira and M. Mota, Eds., Braga, Portugal, September 2008.
  19. Y. Long, Z. Chen, J. L. Duvail, Z. Zhang, and M. Wan, “Electrical and magnetic properties of polyaniline/Fe3O4 nanostructures,” Physica B, vol. 370, no. 1–4, pp. 121–130, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. W. M. De Azevedo, J. M. De Souza, and J. V. De Melo, “Semi-interpenetrating polymer networks based on polyaniline and polyvinyl alcohol-glutaraldehyde,” Synthetic Metals, vol. 100, no. 3, pp. 241–248, 1999. View at Publisher · View at Google Scholar · View at Scopus
  21. D. M. Sherman and T. D. Waite, “Electronic spectra of Fe3+ oxides and oxide hydroxides in the near IR to near UV,” American Mineralogist, vol. 70, no. 11-12, pp. 1262–1269, 1985. View at Google Scholar · View at Scopus
  22. X. H. Wang, Y. H. Geng, L. X. Wang, X. B. Jing, and F. S. Wang, “Thermal stability of polyaniline,” Synthetic Metals, vol. 69, pp. 263–264, 1995. View at Google Scholar
  23. D. Maity, S. -G. Choo, J. Yi, J. Ding, and J. M. Xue, “Synthesis of magnetite nanoparticles via a solvent-free thermal decomposition route,” Journal of Magnetism and Magnetic Materials, vol. 321, no. 9, pp. 1256–1259, 2009. View at Publisher · View at Google Scholar
  24. P. Bénézeth, D. J. Wesolowski, D. A. Palmer, and M. L. MacHesky, “Effect of amines on the surface charge properties of iron oxides,” Journal of Solution Chemistry, vol. 38, no. 7, pp. 925–945, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Hong, D. Xu, P. Gong, J. Yu, H. Ma, and S. Yao, “Covalent-bonded immobilization of enzyme on hydrophilic polymer covering magnetic nanogels,” Microporous and Mesoporous Materials, vol. 109, no. 1–3, pp. 470–477, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. L. Vámos-Vigyázó, “Polyphenol oxidase and peroxidase in fruits and vegetables,” Critical Reviews in Food Science and Nutrition, vol. 15, no. 1, pp. 49–127, 1981. View at Google Scholar · View at Scopus
  27. K. J. Baynton, J. K. Bewtra, N. Biswas, and K. E. Taylor, “Inactivation of horseradish peroxidase by phenol and hydrogen peroxide: a kinetic investigation,” Biochimica et Biophysica Acta, vol. 1206, no. 2, pp. 272–278, 1994. View at Publisher · View at Google Scholar