Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Table 2

Robustness test of proposed method in terms of recovery (%) and RSD (%) for 500 μg/mL CB13 standard (𝑛=3) (MP: mobile phase).

Changes to original method	Theoretical mean (μg)	Peak area	Experimental (μg)	Recovery (%)	RSD
MP proportion (A : B) 65 : 35	500.60	7787498.33	502.83	100.45	0.98
MP proportion (A : B) 75 : 25	500.53	7826118.67	507.95	101.48	1.11
MP pH = 2.70	500.43	7821110.33	507.29	101.37	0.84
MP pH = 3.10	500.50	7811209	505.98	101.09	0.74
Oven temperature −2°C	500.46	7814675	506.44	101.19	1.35
Oven temperature +2°C	500.30	7832734	508.83	101.71	1.04
Flow rate −0.1 mL/min	500.36	7835018,67	509.14	101.75	0.84
Flow rate +0.1 mL/min	500.50	7809528	505.75	101.08	0.87