New Synthetic Pyridine Derivate as Potential Elicitor in Production of Isoflavonoids and Flavonoids in Trifolium pratense L. Suspension Culture

Table 1

Production of isoflavonoids in the suspension culture of Trifolium pratense.

Elicitor concentration (μmol L^−1)	Time after elicitation (hours)	Genistin (mg g^−1 DW)	Daidzein (mg g^−1 DW)	Genistein (mg g^−1 DW)	Formononetin (mg g^−1 DW)	Biochanin A (mg g^−1 DW)
0 (control)	6	4.61±0.03	3.32±0.08	0.31±0.05	3.69±0.09	0.28±0.04
	24	4.61±0.03	3.32±0.08	0.31±0.05	3.69±0.09	0.28±0.04
	48	4.61±0.03	3.32±0.08	0.31±0.05	3.69±0.09	0.28±0.04
	168	6.30±0.05	3.41±0.06	1.19±0.02	3.91±0.06	0.31±0.02
1	6	7.21^∗±0.09	2.61^∗±0.10	0.52±0.10	4.78^∗±0.10	0.29±0.08
	24	8.89^∗±0.04	5.99^∗±0.09	0.88^∗±0.05	1.12^∗±0.08	0.12±0.12
	48	11.60^∗±0.07	8.31^∗±0.04	1.50^∗±0.06	5.83^∗±0.05	0.21±0.07
	168	1.31^∗±0.12	0.32^∗±0.07	0.23^∗±0.04	0.71^∗±0.04	0.09^∗±0.03
10	6	11.49^∗±0.08	2.98^∗±0.04	1.32^∗±0.08	7.31^∗±0.05	0.28±0.09
	24	7.71^∗±0.10	3.13±0.09	0.31±0.02	6.53^∗±0.09	0.00^∗±0.00
	48	7.39^∗±0.05	2.49^∗±0.06	0.48±0.13	3.62±0.11	0.11^∗±0.02
	168	2.20^∗±0.07	0.62^∗±0.10	0.19^∗±0.05	0.42^∗±0.05	0.11^∗±0.05
100	6	5.11^∗±0.04	2.67^∗±0.15	0.42±0.06	0.59^∗±0.07	0.29±0.07
	24	7.19^∗±0.09	2.92^∗±0.08	0.00^∗±0.00	0.72^∗±0.04	0.19±0.04
	48	8.78^∗±0.11	2.42^∗±0.03	0.14^∗±0.05	2.90^∗±0.06	0.11^∗±0.02
	168	0.67^∗±0.07	0.23^∗±0.05	0.32^∗±0.04	1.89^∗±0.05	0.08^∗±0.12

Levels marked with * are statistically significantly different compared with the control each value represents the mean of three samples ± standard deviation.