Research Article

New Synthetic Pyridine Derivate as Potential Elicitor in Production of Isoflavonoids and Flavonoids in Trifolium pratense L. Suspension Culture

Table 2

Production of flavonoids in the suspension culture of Trifolium pratense.

Elicitor concentration (μmol L−1)Time after elicitation (hours)Flavonoid contents (mg g−1 DW)

6 2 . 3 9 ± 0 . 0 7
0 (control)24 2 . 3 9 ± 0 . 0 7
48 2 . 3 9 ± 0 . 0 7
168 2 . 7 1 ± 0 . 0 9

6 4 . 8 7 ± 0 . 0 8
124 2 . 6 7 ± 0 . 0 6
48 2 . 6 9 ± 0 . 0 3
168 2 . 1 2 ± 0 . 0 4

6 5 . 0 5 ± 0 . 1 2
1024 2 . 7 2 ± 0 . 0 5
48 2 . 9 9 ± 0 . 0 2
168 2 . 1 1 ± 0 . 0 9

6 5 . 7 8 ± 0 . 0 3
10024 3 . 5 6 ± 0 . 0 5
48 2 . 4 0 ± 0 . 0 5
168 2 . 2 1 ± 0 . 0 2

Levels marked with * are statistically significantly different compared with the control. Each value represents the mean of three samples ± standard deviation.