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The Scientific World Journal
Volume 2012 (2012), Article ID 759604, 8 pages
http://dx.doi.org/10.1100/2012/759604
Research Article

Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

1Institute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech Republic
2Kalmykian State University, Elista, Russia
3Cardiovascular Research Centre, Prague, Czech Republic

Received 27 October 2011; Accepted 19 December 2011

Academic Editor: Masumi Akita

Copyright © 2012 Kateřina Mikulíková et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I ( 𝛼 1 ) (from bovine) and collagen type I ( 𝛼 2 ) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.