Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach
Table 2
Putative identity of the proteins identified by mass spectrometry (LC-MS/MS). Hits with the lowest E-value are represented which are proteins with the biggest homology to the peptides obtained from LC-MS/MS. The emPAI (Exponentially Modified Protein Abundance Index) value offers approximate, label-free, relative quantitation of the proteins in a mixture based on protein coverage by the peptide matches in a database search result [23].
Spots [EST Accession Number]
Putative identity
emPAI score
WSM 2 [gi164576479]
Peptidylprolyl isomerase B/cyclophilin B
0,28
WSM 2 [gi164584223]
Gigasin-2
0,70
WSM 2 [gi164584724]
Gigasin-2
1,47
WSM 3 [gi164576479]
Peptidylprolyl isomerase B / cyclophilin B
0,13
WSM 3 [gi164584223]
Gigasin-2
0,70
WSM 3 [gi164584724]
Gigasin-2
1,47
WSM 4 [gi115724273]
Putative serine/threonine-specific protein kinase
0,11
WSM 4 [gi164582888]
Gigasin-2 like
1,25
WSM 4 [gi168810136]
Gigasin-2 like
0,43
WSM 7 [gi164582888]
Gigasin-2 like
0,97
WSM 7 [gi168810136]
Gigasin-2 like
0,71
WSM 8 [gi14581004]
PLCPI = cysteine proteinase inhibitor/cystatin A (stefin A)
0,16
WSM 8 [gi164570244]
Cystatin A2
0,62
WSM 13 [gi14581004]
PLCPI = cysteine proteinase inhibitor/cystatin A (stefin A)
