Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2012, Article ID 859381, 4 pages
http://dx.doi.org/10.1100/2012/859381
Research Article

Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)

1Department of Horticultural Science, College of Industrial Sciences, Kongju National University, 1 Daehoe-ri, Yesan-kun, Chungnam 340-720, Republic of Korea
2Department of Well-Being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do 540-742, Republic of Korea
3Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 99 Daehangno, Yuseong-gu, Daejeon 305-764, Republic of Korea

Received 2 August 2012; Accepted 23 September 2012

Academic Editors: Y. Shoyama and R. B. Widelitz

Copyright © 2012 Soo Cheon Chae et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG, CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl2 the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl2 at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol in S. speciosa for plant regenerations.