Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro

Table 1

Primer sequences for target cDNAs.

Target cDNA	Primer sequence
GAPDH (200 bp)	Upstream: 5′-TCC ATG ACA ACT TTG GTA TCG-3′Downstream: 5′-ATG AGT CCT TCC ACG ATA CCA-3′
Osteopontin (220 bp)	Upstream: 5′-CTC AGA GGA GAA GGC GCA TTG-3′Downstream: 5′-TCT CTG CAT GGT CTC CGT CGT -3′
Osteonectin (225 bp)	Upstream: 5′-GTC CTG GTC ACC TTG TAC GAG-3′Downstream: 5′-GGG ACA GGT ACC CAT CAA TCG -3′
Osteocalcin (175 bp)	Upstream: 5′-AAG GTG GTG AAT AGA CTC CG-3′Downstream: 5′-AAA CGG TGG TGC CAT AGA TG-3′
Type I collagen (181 bp)	Upstream: 5′-TGC CGT GAC CTC AAG ATG T-3′Downstream: 5′-TGG GGT TTG GGC TGA TGT A-3′
DMP-1 (163 bp)	Upstream: 5′-AGC ATT CCT CTA ATC CAG TT-3′Downstream: 5′-CTG ATC TAA ACA AGT GCC AT-3′