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The Scientific World Journal
Volume 2012, Article ID 906873, 5 pages
Clinical Study

Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus

1Pharmaceutical Sciences Post Graduate Program, State University of Ponta Grossa (UEPG), General Carlos Cavalcanti Avenue, 4748 Uvaranas, 84030-900 Ponta Grossa, PR, Brazil
2Department of Pharmacy, State University in the Paraná Midwestern (UNICENTRO), CEDETEG Campus, 85040-080 Guarapuava, PR, Brazil
3County Health Department, Expert Assistance Service (EAS), City Hall, 84051-900 Ponta Grossa, PR, Brazil
4Department of Physiotherapy, State University Paulista (UNESP), Presidente Prudente Campus, 19060-900 Presidente Prudente, SP, Brazil

Received 1 September 2012; Accepted 8 November 2012

Academic Editors: A. Banerjee and E. L. Hooghe-Peters

Copyright © 2012 Danielle Cristyane Kalva Borato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Considering that counting the percentage of CD4 T lymphocytes can add prognostic information regarding patients infected with HIV, the aim of this study was to evaluate the percentage values of CD4+ T lymphocytes from 81 patients determined by flow cytometry and estimated by flow cytometry in conjunction with a hematology counter. Means were compared through the Student's t-test. Pearson's correlation was determined, and the agreement between results was tested by Bland-Altman. The level of significance was . It was found a significantly higher mean difference between the relative values of CD4+ T lymphocytes to the hematologic counter ( ), for all strata studied. Positive and significant correlations ( ) were found between the strata CD4 < 200 cells/mL ( ), between 200 and 500 cells/mL ( ), and >500 cells/mL ( ). The limits of agreement were % for the stratum of CD4 < 200 cells/mL, approximately % for the stratum of CD4 between 200 and 500 cells/mL, and approximately % for the stratum > 500 cells/mL. The differences in the percentages of CD4+ T lymphocytes obtained by different methodologies could lead to conflict when used in clinical decisions related to the treatment and care of people infected with HIV.