An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Target gene</td><td align="center">Primer name</td><td align="center">Sequence (5′-3′)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">HA</td><td align="center">H5-1/H5-3</td><td align="center">GCCATTCCACAACATACACCC/CTCCCCTGCTCATTGCTATG</td></tr><tr><td align="left">M</td><td align="center">M30F/M264R</td><td align="center">TTCTAACCGAGGTCGAAACG/ACAAAGCGTCTACGCTGCAG</td></tr><tr><td align="left">N1</td><td align="center">N1-1/N1-2</td><td align="center">TTGCTTGGTCGGCAAGTGC/CCAGTCCACCCATTTGGATCC</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Primer pairs recommended by WHO for H5N1.

The Scientific World Journal

tab2

Table 2

Table 2: An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues 