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The Scientific World Journal
Volume 2012 (2012), Article ID 913412, 8 pages
Research Article

Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System

Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari 4818168984, Iran

Received 25 October 2011; Accepted 22 December 2011

Academic Editor: Xavier Santarelli

Copyright © 2012 Sayed Yahya Kazemi and Seyed Mohammad Abedirad. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP). The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with 𝑘 𝑄 values of 3 . 1 7 × 1 0 5 and 3 . 7 × 1 0 5 M − 1 in the quencher concentration range of 1 . 5 × 1 0 − 6 to 1 . 5 × 1 0 − 5  M for human serum albumin (HSA) and bovine serum albumin (BSA), respectively.