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The Scientific World Journal
Volume 2012 (2012), Article ID 940541, 9 pages
http://dx.doi.org/10.1100/2012/940541
Research Article

Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

1Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
2Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran

Received 25 October 2011; Accepted 8 December 2011

Academic Editor: Carmina Montoliu

Copyright © 2012 Amir M. Ramezani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb3+-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb3+-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb3+-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: p H = 7 . 8 , [Tb3+] = 8 . 5 × 1 0 5  mol L−1, [Dano] = 1 . 5 × 1 0 4  mol L−1. The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0 . 2 × 1 0 6 1 . 3 × 1 0 6  mol L−1, 0 . 2 × 1 0 6 1 . 4 × 1 0 6  mol L−1, and 0 . 2 × 1 0 6 1 × 1 0 6  mol L−1, respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8 . 7 × 1 0 8  mol L−1, 6 . 2 × 1 0 8  mol L−1, and 8 . 1 × 1 0 8  mol L−1, respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.