Research Article
Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe
Table 2
Interference effect on the enhanced fluorescence intensity(ΔF%).
| | Interfere substance | Concentration of interfere substance (×10−3 mol L−1) | Tolerance ratio (species/HSA) | Chang of |
| | Na+(Cl−) | 5 | 5000 | 1.3 | | Fe3+ (Cl−) | 0.005 | 5 | −4.2 | | K+ (CL−) | 3 | 3000 | −2.3 | | (Cl−) | 4.2 | 4200 | −4.7 | | Saccharose | 1 | 1000 | 3.7 | | Glycine | 1 | 1000 | −2.4 | | Mn2+ (Cl−) | 0.05 | 50 | −3.8 | | ctDNA | 0.00075 | 0.75 | 3.0 | | Al3+ (Cl−) | 0.005 | 5 | −4.9 | | Ba2+ (Cl−) | 1.4 | 1400 | −3.4 | | Ca2+ (Cl−) | 1.15 | 1150 | −4.2 | | Na+ () | 1.19 | 1900 | −3.7 |
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Analytical conditions: [Tb3+]: 8.5 × 10−5 mol L−1; [Dano]: 1.5 × 10−4 mol L−1; [HSA]: 1 × 10−6 mol L−1; pH = 7.8.
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