Research Article
Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe
Table 4
Intra-assay precision and accuracy of calibration standards.
| | Analyte name | Nominal concentration (mol L−1) () | Found concentration
(mol L−1) () | Precision (RSD%) | Accuracy (RE %) |
| | BSA | 0.3 × 10−6 | 0.286 × 10−6 | 2.78 | −4.5 | | 0.5 × 10−6 | 0.489 × 10−6 | 3.91 | −2.2 | | 0.7 × 10−6 | 0.714 × 10−6 | 3.97 | 1.9 | | 1.0 × 10−6 | 0.992 × 10−6 | 0.50 | −0.8 | | 1.2 × 10−6 | 1.194 × 10−6 | 2.78 | −0.5 |
| | HSA | 0.3 × 10−6 | 0.308 × 10−6 | 2.85 | 2.9 | | 0.5 × 10−6 | 0.510 × 10−6 | 3.19 | 2.1 | | 0.7 × 10−6 | 0.713 × 10−6 | 3.14 | 1.9 | | 1.0 × 10−6 | 0.994 × 10−6 | 1.53 | −0.5 | | 1.2 × 10−6 | 1.185 × 10−6 | 1.44 | −1.2 |
| | HSA in plasma | 0.3 × 10−6 | 0.277 × 10−6 | 4.13 | −7.6 | | 0.5 × 10−6 | 0.519 × 10−6 | 3.94 | 3.7 | | 0.7 × 10−6 | 0.711 × 10−6 | 5.08 | 1.6 | | 0.8 × 10−6 | 0.791 × 10−6 | 4.23 | −1.0 | | 1.0 × 10−6 | 0.959 × 10−6 | 3.58 | −4.1 |
|
|
