Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2012, Article ID 979218, 6 pages
http://dx.doi.org/10.1100/2012/979218
Research Article

Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast

Institute of Biotechnology, Vilnius University, Graiciuno 8, Vilnius, Lithuania

Received 24 October 2011; Accepted 21 December 2011

Academic Editors: V. Dvornyk, S. Y. Morozov, and R. D. Possee

Copyright © 2012 Milda Norkiene and Alma Gedvilaite. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. Angov, “Codon usage: nature's roadmap to expression and folding of proteins,” Biotechnology Journal, vol. 6, no. 6, pp. 650–659, 2011. View at Publisher · View at Google Scholar
  2. J. F. Kane, “Effects if rare codon clusters on high-level expression of heterologous proteins in Echerichia coli,” Current Opinion in Biotechnology, vol. 6, no. 5, pp. 494–500, 1995. View at Publisher · View at Google Scholar · View at Scopus
  3. E. Goldman, A. H. Rosenberg, G. Zubay, and F. W. Studier, “Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Echerichia coli,” Journal of Molecular Biology, vol. 245, no. 5, pp. 467–473, 1995. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Kanaya, Y. Yamada, Y. Kudo, and T. Ikemura, “Bacillus subtilis tRNAs: gene expression level and species-specific diversity of codon usage based on multivariate analysis,” Gene, vol. 238, no. 1, pp. 143–155, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Gustafsson, S. Govindarajan, and J. Minshull, “Codon bias and heterologous protein expression,” Trends in Biotechnology, vol. 22, no. 7, pp. 346–353, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. Z. Zhou, P. Schnake, L. Xiao, and A. A. Lal, “Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization,” Protein Expression and Purification, vol. 34, no. 1, pp. 87–94, 2004. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Elf, D. Nilsson, T. Tenson, and M. Ehrenberg, “Selective charging of tRNA isoacceptors explains patterns of codon usage,” Science, vol. 300, no. 5626, pp. 1718–1722, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. K. A. Dittmar, M. A. Sørensen, J. Elf, M. Ehrenberg, and T. Pan, “Selective charging of tRNA isoacceptors induced by amino-acid starvation,” EMBO Reports, vol. 6, no. 2, pp. 151–157, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. E. Angov, C. J. Hillier, R. L. Kincaid, and J. A. Lyon, “Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host,” PLoS ONE, vol. 3, no. 5, Article ID e2189, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. J. B. Plotkin and G. Kudla, “Synonymous but not the same: the causes and consequences of codon bias,” Nature Reviews Genetics, vol. 12, no. 1, pp. 32–42, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Zhou, W. J. Liu, S. W. Peng, X. Y. Sun, and I. Frazer, “Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability,” Journal of Virology, vol. 73, no. 6, pp. 4972–4982, 1999. View at Google Scholar · View at Scopus
  12. C. Leder, J. A. Kleinschmidt, C. Wiethe, and M. Müller, “Enhancement of capsid gene expression: preparing the human papillomavirus type 16 major structural gene l1 for DNA vaccination purposes,” Journal of Virology, vol. 75, no. 19, pp. 9201–9209, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Biemelt, U. Sonnewald, P. Galmbacher, L. Willmitzer, and M. Müller, “Production of human papillomavirus type 16 virus-like particles in transgenic plants,” Journal of Virology, vol. 77, no. 17, pp. 9211–9220, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. J. Sambrook and D. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 2001.
  15. R. Kirnbauer, J. Taub, H. Greenstone et al., “Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles,” Journal of Virology, vol. 67, no. 12, pp. 6929–6936, 1993. View at Google Scholar · View at Scopus
  16. K. Sasnauskas, O. Buzaite, F. Vogel et al., “Yeast cells allow high-level expression and formation of polyomavirus-like particles,” Biological Chemistry, vol. 380, no. 3, pp. 381–386, 1999. View at Google Scholar · View at Scopus
  17. K. Sasnauskas, R. Jomantiene, A. Januska, E. Lebediene, J. Lebedys, and A. Janulaitis, “Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae,” Gene, vol. 122, no. 1, pp. 207–211, 1992. View at Publisher · View at Google Scholar · View at Scopus
  18. M. E. Schmitt, T. A. Brown, and B. L. Trumpower, “A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae,” Nucleic Acids Research, vol. 18, no. 10, pp. 3091–3092, 1990. View at Google Scholar · View at Scopus
  19. A. Gedvilaite, A. Zvirbliene, J. Staniulis, K. Sasnauskas, D. H. Krüger, and R. Ulrich, “Segments of puumala hantavirus nucleocapsid protein inserted into chimeric polyomavirus-derived virus-like particles induce a strong immune response in mice,” Viral Immunology, vol. 17, no. 1, pp. 51–68, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. A. Hoekema, R. A. Kastelein, M. Vasser, and H. A. de Boer, “Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression,” Molecular and Cellular Biology, vol. 7, no. 8, pp. 2914–2924, 1987. View at Google Scholar · View at Scopus
  21. J. L. Bennetzen and B. D. Hall, “Codon selection in yeast,” Journal of Biological Chemistry, vol. 257, no. 6, pp. 3026–3031, 1982. View at Google Scholar · View at Scopus