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The Scientific World Journal
Volume 2012, Article ID 979682, 7 pages
http://dx.doi.org/10.1100/2012/979682
Research Article

Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks

1Department of Horticulture, Faculty of Agriculture, Eskisehir Osmangazi University, 26160 Eskisehir, Turkey
2Department of Horticulture, Faculty of Agriculture, Uludag University, 16059 Bursa, Turkey

Received 24 October 2011; Accepted 12 December 2011

Academic Editor: David P. Horvath

Copyright © 2012 Ece Turhan and Sergul Ergin. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA) and nonacclimated (NA) stages. Bark tissues subjected to 4°C and −5°C injured to a limited extent in both stages. However, more than 50% injury occurred by temperatures equal to or colder than −15°C only in NA period. Total soluble sugar (TSS), reducing sugars, and sucrose contents were higher in CA than those in NA stages in all samples. The activities of acid invertase (EC 3.2.1.26) and sucrose synthase (SS) (EC 2.4.2.13) enzymes were higher in NA stage than those in CA stage. Considering the rootstocks, reducing sugars were higher in both cultivars grafted on Gisela 5 whereas sucrose contents were higher in both cultivars grafted on Mazzard. However, the enzyme activities of both cultivars were higher on Mazzard rootstock than on Gisela 5. In conclusion, cold hardiness of sweet cherry graft combinations was suggested by increasing their TSS, reducing sugars, and sucrose contents significantly in the CA stage. Moreover, acid invertase and SS are down regulated during cold acclimation. Indeed the results suggested that Mazzard is more cold-hardy rootstock than Gisela 5.