(a) Primer extension
(b) Western blotting
Figure 2: OpaR-dependent expression of Hcp1. (a) Primer extension. An oligonucleotide primer was designed to be complementary to the RNA transcript of opaR. The primer extension products were analyzed with 8 M urea—6% acrylamide sequencing gel. Lanes C, T, A, and G represent the Sanger sequencing reactions. The transcription start site of hcp1 was underlined in the DNA sequences. (b) Immunoblot analysis.The Hcp1 production was detected in WT or ΔopaR, while His-Hcp1 was used as positive control.