Figure 3: OpaR-dependent promoter activity of hcp1. A promoter-proximal region 504 bp upstream to 40 bp downstream of hcp1 was cloned into pRBR309 containing a promoterless lacZ reporter gene and then transformed into WT or ΔopaR to determine the β-galactosidase activity in cellular extracts. Shown are the hcp1 promoter activities (Miller units) in WT or ΔopaR.