Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells
Figure 4
Chromatin immunoprecipitation analysis of FUT1 promoter in DLD-1 cells. Chromatin immunoprecipitation (CHIp) analysis for the Elk-1 binding site region within FUT1 promoter in DLD-1 cells. (a) Primers designed for the 5′-flanking region of FUT1 (Primer A: −140 to −41, Primer B: −534 to −424). (b) DLD-1 cells transfected with pHTC/Elk-1 vector were precipitated and amplified by PCR with specific primers (see (a)) as described in Section 2. gDNA: positive control; PCR amplification for fragmented and nonprecipitated genomic DNA from DLD-1 cells 24 h aftertransfection of Halo-tagged Elk-1 gene. Halo ip: PCR amplification for fragmented and precipitated genomic DNA with Halo resin from the DLD-1 cells 24 h aftertransfection of Halo-tagged Elk-1 gene.
