HL cells were plated, grown, and infected with C. pneumoniae at MOI 1. Additions of the Roquefort cheese protein extract with final concentrations of 0.12, 0.25, and 0.50 mg/mL were performed simultaneously with the bacterial pathogen inoculation. The cell monolayers were harvested after 72 h incubation at 35°C in 5% CO2 and immunofluorescent staining conducted as described in Section 2. Inhibition of C. pneumonia growth by roquefort protein extract ((a) uninfected cells; (b) infected cells; (c1), (c2), and (c3) infected cells grown in the presence of 0.12, 0.25, and 0.50 mg/mL Roquefort protein extract, resp.).
