Research Article
Association of FAS and FAS Ligand Genes Polymorphism and Risk of Systemic Lupus Erythematosus
Table 1
The primer sequences, annealing temperature, restriction enzymes, and fragments size of FAS and FASL polymorphisms.
| Target sequence | Primer sequence | Annealing temperature | Restriction enzyme | PCR product | RFLP fragments |
| FAS A-670G rs1800682 | Forward: 5′-CTACCTAAGAGCTATCTACCGTTC-3′ Reverse: 5′-GGCTGTCCATGTTGTGGCTGC-3′[29] | 61°C | MvaI (BstNI) | 223 | A: 223 G: 44, 189 | FASL C-844T rs763110 | Forward: 5′-CAGCTACTCGGAGGCCAAG-3′ Reverse: 5′-GCTCTGAGGGGAGAGACCAT-3′[19] | 63°C | BseMI (BsrDI) | 401 | T: 401 C: 223, 168 |
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