Online Monitoring Oxidative Products and Metabolites of Nicotine by Free Radicals Generation with Fenton Reaction in Tandem Mass Spectrometry

Figure 3

Electrodeless electrochemical oxidation (EEO) method coupled with UHPLC-MS/MS showed the spectra of the nicotine and its derivatives labeled with I–IX. The spectra displayed (a) base peak chromatogram and fragmentation pattern of nicotine (b), cotinine (c), trans-3′-hydroxyl cotinine (d), nornicotine (e), nicotin-N-oxide (f), norcotinine (g), 4-oxo-4-(3-pyridyl)-butanoic acid (h), 4-hydroxy-4-(3-pyridyl)-butanoic acid (i), cotinine-N′-oxide (j).

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