Essential Roles of PKA, iNOS, CD95/CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents
PGE2 suppresses eosinophilopoiesis by activating a caspase-dependent mechanism. Bone-marrow cultures were established in the presence of IL-5, alone or associated with PGE2, caspase inhibitor zVAD-fmk, or both. All cultures were maintained for 7 days, except for (b). All agents were added at the beginning of the culture, except for (c). Data (mean + SEM; ) are the numbers of EPO+ cells recovered at the end of the culture ((a)–(c)) or the % eosinophilopoiesis suppression in the indicated conditions, where inhibitors were used to prevent this suppression (d). Significant differences relative to the respective IL-5 controls: , , , .
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.