Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2013, Article ID 241686, 7 pages
http://dx.doi.org/10.1155/2013/241686
Research Article

Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12

Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SD, Brazil

Received 15 May 2013; Accepted 29 June 2013

Academic Editors: C. DebRoy, D. Endoh, and B. I. Yoon

Copyright © 2013 Bruna Rocha Passos Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. K. Estes and A. Z. Kapikian, “Rotaviruses,” in Fields Virology, B. N. Fields, P. M. Knipe, and P. M. Howley, Eds., pp. 1917–1973, Lippincott Williams & Wilkins, Philadelphia, Pa, USA, 5th edition, 2007. View at Google Scholar
  2. A. A. Alfieri, A. F. Alfieri, and E. A. Beuttemmüller, “Rotavirose suína: tópicos sobre etiologia, infecção e controle,” Semina: Ciências Agrárias, vol. 20, no. 1, pp. 90–97, 1999. View at Google Scholar
  3. Paho: Pan American Health Organization, “Rotaviral gastroenteritis,” in Zoonoses and Communicable Diseases Common to Man and Animals: Chlamydioses, Rickettsioses and Viroses, pp. 286–294, PAHO, Washington, DC, USA, 3rd edition, 2001. View at Google Scholar
  4. L. J. Saif and B. Jiang, “Nongroup A rotaviruses of humans and animals,” Current Topics in Microbiology and Immunology, vol. 185, pp. 339–371, 1994. View at Google Scholar · View at Scopus
  5. V. Martella, K. Bányai, J. Matthijnssens, C. Buonavoglia, and M. Ciarlet, “Zoonotic aspects of rotaviruses,” Veterinary Microbiology, vol. 140, no. 3-4, pp. 246–255, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. A. M. Q. King, M. J. Adams, E. B. Carstens, and E. J. Lefkowitz, Eds., Virus Taxonomy: Classification and Nomenclature of Viruses, Elsevier Academic Press, San Diego, Calif, USA, 9th edition, 2012.
  7. P. Schuck, Z. Taraporewala, P. McPhie, and J. T. Patton, “Rotavirus nonstructural protein NSP2 self-assembles into octamers that undergo ligand-induced conformational changes,” The Journal of Biological Chemistry, vol. 276, no. 13, pp. 9679–9687, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. Z. F. Taraporewala and J. T. Patton, “Identification and characterization of the helix-destabilizing activity of rotavirus nonstructural protein NSP2,” Journal of Virology, vol. 75, no. 10, pp. 4519–4527, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. T. Bar-Magen, E. Spencer, and J. T. Patton, “An ATPase activity associated with the rotavirus phosphoprotein NSP5,” Virology, vol. 369, no. 2, pp. 389–399, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. L. Hu, D. C. Chow, J. T. Patton, T. Palzkill, M. K. Estes, and B. V. V. Prasad, “Crystallographic analysis of rotavirus NSP2-RNA complex reveals specific recognition of 5GG sequence for RTPase activity,” Journal of Virology, vol. 86, no. 19, pp. 10547–10557, 2012. View at Publisher · View at Google Scholar
  11. I. Afrikanova, E. Fabbretti, M. C. Miozzo, and O. R. Burrone, “Rotavirus NSP5 phosphorylation is up-regulated by interaction with NSP2,” Journal of General Virology, vol. 79, no. 11, pp. 2679–2686, 1998. View at Google Scholar · View at Scopus
  12. M. Berois, C. Sapin, I. Erk, D. Poncet, and J. Cohen, “Rotavirus nonstructural protein NSP5 interacts with major core protein VP2,” Journal of Virology, vol. 77, no. 3, pp. 1757–1763, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Eichwald, G. Jacob, B. Muszynski, J. E. Allende, and O. R. Burrone, “Uncoupling substrate and activation functions of rotavirus NSP5: phosphorylation of Ser-67 by casein kinase 1 is essential for hyperphosphorylation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 46, pp. 16304–16309, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. C. Eichwald, J. F. Rodriguez, and O. R. Burrone, “Characterization of rotavirus NSP2/NSP5 interactions and the dynamics of viroplasm formation,” Journal of General Virology, vol. 85, no. 3, pp. 625–634, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. J. J. Carreño-Torres, M. Gutiérrez, C. F. Arias, S. López, and P. Isa, “Characterization of viroplasm formation during the early stages of rotavirus infection,” Virology Journal, vol. 7, article 350, 2010. View at Publisher · View at Google Scholar
  16. A. Sen, D. Agresti, and E. R. Mackow, “Hyperphosphorylation of the rotavirus NSP5 protein is independent of serine 67, NSP2, or the intrinsic insolubility of NSP5 and is regulated by cellular phosphatases,” Journal of Virology, vol. 80, no. 4, pp. 1807–1816, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. M. A. Torres-Vega, R. A. González, M. Duarte, D. Poncet, S. López, and C. F. Arias, “The C-terminal domain of rotavirus NSP5 is essential for its multimerization, hyperphosphorylation and interaction with NSP6,” Journal of General Virology, vol. 81, no. 3, pp. 821–830, 2000. View at Google Scholar · View at Scopus
  18. M. Samaniego-Hernández, A. León-Rodríguez, R. Aparicio-Fabre, C. Arias-Ortiz, and A. P. Barba de La Rosa, “Expression and purification of rotavirus proteins NSP5 and NSP6 in Escherichia coli,” Cell Biochemistry and Biophysics, vol. 44, no. 3, pp. 336–341, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. E. W. Rainsford and M. A. McCrae, “Characterization of the NSP6 protein product of rotavirus gene 11,” Virus Research, vol. 130, no. 1-2, pp. 193–201, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Matthijnssens, M. Ciarlet, S. M. Mcdonald et al., “Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG),” Archives of Virology, vol. 156, no. 8, pp. 1397–1413, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. J. Matthijnssens, M. Rahman, V. Martella et al., “Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission,” Journal of Virology, vol. 80, no. 8, pp. 3801–3810, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Tsugawa and Y. Hoshino, “Whole genome sequence and phylogenetic analyses reveal human rotavirus G3P[3] strains Ro1845 and HCR3A are examples of direct virion transmission of canine/feline rotaviruses to humans,” Virology, vol. 380, no. 2, pp. 344–353, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. P. Khamrin, N. Maneekarn, R. Malasao et al., “Genotypic linkages of VP4, VP6, VP7, NSP4, NSP5 genes of rotaviruses circulating among children with acute gastroenteritis in Thailand,” Infection, Genetics and Evolution, vol. 10, no. 4, pp. 467–472, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. N. Cook, J. Bridger, K. Kendall, M. I. Gomara, L. El-Attar, and J. Gray, “The zoonotic potential of rotavirus,” Journal of Infection, vol. 48, no. 4, pp. 289–302, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Steyer, M. Poljšak-Prijatelj, D. Barlič-Maganja, and J. Marin, “Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment,” Journal of General Virology, vol. 89, no. 7, pp. 1690–1698, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. V. Martella, M. Ciarlet, R. Baselga et al., “Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses,” Virology, vol. 337, no. 1, pp. 111–123, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Mukherjee, D. Dutta, S. Ghosh et al., “Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission,” Archives of Virology, vol. 154, no. 5, pp. 733–746, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. A. N. B. Salem, A. C. Sergei, P. B. Olga, G. A. Olga, A. Mahjoub, and B. P. Larissa, “Multiplex nested RT-PCR for the detection of porcine enteric viruses,” Journal of Virological Methods, vol. 165, no. 2, pp. 283–293, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. T. A. Hall, “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT,” Nucleic Acids Symposium Series, vol. 41, pp. 95–98, 1999. View at Google Scholar
  30. M. A. Larkin, G. Blackshields, N. P. Brown et al., “Clustal W and Clustal X version 2.0,” Bioinformatics, vol. 23, no. 21, pp. 2947–2948, 2007. View at Publisher · View at Google Scholar · View at Scopus
  31. S. F. Altschul, W. Gish, W. Miller, E. W. Myers, and D. J. Lipman, “Basic local alignment search tool,” Journal of Molecular Biology, vol. 215, no. 3, pp. 403–410, 1990. View at Publisher · View at Google Scholar · View at Scopus
  32. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods,” Molecular Biology and Evolution, vol. 28, no. 10, pp. 2731–2739, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. J. T. Patton, L. Salter-Cid, A. Kalbach, E. A. Mansell, and M. Kattoura, “Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35,” Virology, vol. 192, no. 2, pp. 438–446, 1993. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Kumar, H. Jayaram, R. Vasquez-Del Carpio et al., “Crystallographic and biochemical analysis of rotavirus NSP2 with nucleotides reveals a nucleoside diphosphate kinase-like activity,” Journal of Virology, vol. 81, no. 22, pp. 12272–12284, 2007. View at Publisher · View at Google Scholar · View at Scopus
  35. P. H. Sotelo, M. Schümann, E. Krause, and J. Chnaiderman, “Analysis of rotavirus non-structural protein NSP5 by mass spectrometry reveals a complex phosphorylation pattern,” Virus Research, vol. 149, no. 1, pp. 104–108, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. F. Gregori, C. A. R. Rosales, P. E. Brandão, R. M. Soares, and J. A. Jerez, “Diversidade genotípica de rotavírus suínos no Estado de São Paulo,” Pesquisa Veterinária Brasileira, vol. 29, pp. 707–712, 2009. View at Google Scholar
  37. R. C. Linares, A. F. Barry, A. F. Alfieri et al., “Frequency of group A rotavirus in piglet stool samples from non-vaccinated Brazilian pig herds,” Brazilian Archives of Biology and Technology, vol. 52, pp. 63–68, 2009. View at Publisher · View at Google Scholar
  38. K. C. Médici, A. F. Barry, A. F. Alfieri, and A. A. Alfieri, “Porcine rotavirus groups A, B, and C identified by polymerase chain reaction in a fecal sample collection with inconclusive results by polyacrylamide gel electrophoresis,” Journal of Swine Health and Production, vol. 19, no. 3, pp. 146–150, 2011. View at Google Scholar · View at Scopus
  39. J. A. Matthijnssens, M. Ciarlet, E. Heiman et al., “Full genome-based classification of rotaviruses reveals a common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains,” Journal of Virology, vol. 82, no. 7, pp. 3204–3219, 2008. View at Publisher · View at Google Scholar · View at Scopus
  40. E. M. Heiman, S. M. McDonald, M. Barro, Z. F. Taraporewala, T. Bar-Magen, and J. T. Patton, “Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions,” Journal of Virology, vol. 82, no. 22, pp. 11106–11116, 2008. View at Publisher · View at Google Scholar · View at Scopus
  41. M. D. Esona, A. Geyer, N. Page et al., “Genomic characterization of human rotavirus G8 strains from the African rotavirus network: relationship to animal rotaviruses,” Journal of Medical Virology, vol. 81, no. 5, pp. 937–951, 2009. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Ghosh, N. Kobayashi, S. Nagashima et al., “Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172,” Virus Genes, vol. 40, no. 3, pp. 382–388, 2010. View at Publisher · View at Google Scholar · View at Scopus
  43. M. L. Dyall-Smith, T. C. Ellemant, P. A. Hoyne, I. H. Holmes, and A. A. Azad, “Cloning and sequence of UK bovine rotavirus gene segment 7: marked sequence homology with simban rotavirus gene segment 8,” Nucleic Acids Research, vol. 11, no. 10, pp. 3351–3362, 1983. View at Publisher · View at Google Scholar · View at Scopus
  44. K. Rushlow, A. Mcnab, K. Olson, F. Maxwell, I. Maxwell, and G. Stiegler, “Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9,” Nucleic Acids Research, vol. 16, no. 1, pp. 367–368, 1988. View at Publisher · View at Google Scholar · View at Scopus
  45. K. Taniguchi and S. Urasawa, “Diversity in rotavirus genomes,” Seminars in Virology, vol. 6, no. 2, pp. 123–131, 1995. View at Publisher · View at Google Scholar · View at Scopus
  46. G. I. Parra, G. Vidales, J. A. Gomez, F. M. Fernandez, V. Parreño, and K. Bok, “Phylogenetic analysis of porcine rotavirus in Argentina: increasing diversity of G4 strains and evidence of interspecies transmission,” Veterinary Microbiology, vol. 126, no. 1–3, pp. 243–250, 2008. View at Publisher · View at Google Scholar · View at Scopus