Intranuclear Crosstalk between Extracellular Regulated Kinase1/2 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation
Figure 1
Kinetics of LIF-induced phosphorylation of STAT3 and ERK1/2 in JEG-3 choriocarcinoma cells. (a) Representative Western blot of lysates from cells starved in serum-free medium and subsequently stimulated with 10 ng/mL LIF. The bands of all blots () have been scanned for density analysis. The density of bands from phosphorylated proteins ((b): phospho-STAT3 (ser727); (c): phospho-ERK1/2; (d): phospho-STAT3 (tyr705)) has been normalized against the nonphosphorylated form. The so obtained relative density at 10 min LIF stimulation has been defined as “1” and the other values have been calculated, respectively. Bars show means, error bars show standard error. *Indicates when compared with value at 0 min LIF stimulation (Student’s -test). Results of an analysis of correlation after Pearson of the respective kinetics is displayed in the left upper corner of each figure. indicates a significant correlation between stimulation time and band intensity.
