Serum N-Glycan Profiling Predicts Prognosis in Patients Undergoing Hemodialysis
Table 1
Human serum N-glycans targeted in this study.
Peak no.
Composition
Peak no.
Composition
1
1362.48
(Hex)2 + (Man)3(GlcNAc)2
30
2260.8349
(Hex)1(HexNAc)3(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
2
1387.51
(HexNAc)1(dHex)1 + (Man)3(GlcNAc)2
31
2263.8345
(Hex)2(HexNAc)3(dHex)2 + (Man)3(GlcNAc)2
3
1403.51
(Hex)1(HexNAc)1 + (Man)3(GlcNAc)2
32
2276.8298
(Hex)2(HexNAc)3(NeuAc)1 + (Man)3(GlcNAc)2
4
1444.53
(HexNAc)2 + (Man)3(GlcNAc)2
33
2279.8294
(Hex)2(HexNAc)3(NeuAc)1 + (Man)3(GlcNAc)2
5
1524.53
(Hex)3 + (Man)3(GlcNAc)2
34
2336.8509
(Hex)3(HexNAc)4 + (Man)3(GlcNAc)2
6
1549.57
(Hex)1(HexNAc)1(dHex)1 + (Man)3(GlcNAc)2
35
2348.8619
Internal standard (BOA-labeled A2-amide)
7
1590.59
(HexNAc)2(dHex)1 + (Man)3(GlcNAc)2
36
2378.8615
(Hex)2(HexNAc)2(NeuAc)2 + (Man)3(GlcNAc)2
8
1606.59
(Hex)1(HexNAc)2 + (Man)3(GlcNAc)2
37
2406.8928
(Hex)1(HexNAc)3(dHex)2(NeuAc)1 + (Man)3(GlcNAc)2
9
1647.61
(HexNAc)3 + (Man)3(GlcNAc)2
38
2419.8932
(Hex)1(HexNAc)3(NeuAc)2 + (Man)3(GlcNAc)2
10
1686.59
(Hex)4 + (Man)3(GlcNAc)2
39
2422.8877
(Hex)2(HexNAc)3(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
11
1708.62
(Hex)1(HexNAc)1(NeuAc)1 + (Man)3(GlcNAc)2
40
2438.8826
(Hex)3(HexNAc)3(NeuAc)1 + (Man)3(GlcNAc)2
12
1752.64
(Hex)1(HexNAc)2(dHex)1 + (Man)3(GlcNAc)2
41
2482.9088
(Hex)3(HexNAc)4(dHex)1 + (Man)3(GlcNAc)2
13
1768.64
(Hex)2(HexNAc)2 + (Man)3(GlcNAc)2
42
2498.9037
(Hex)4(HexNAc)4 + (Man)3(GlcNAc)2
14
1793.67
(HexNAc)3(dHex)1 + (Man)3(GlcNAc)2
43
2524.9194
(Hex)2(HexNAc)2(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2
15
1809.67
(Hex)1(HexNAc)3 + (Man)3(GlcNAc)2
44
2584.9405
(Hex)3(HexNAc)3(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
16
1848.64
(Hex)5 + (Man)3(GlcNAc)2
45
2727.9988
(Hex)2(HexNAc)3(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2
17
1854.68
(Hex)1(HexNAc)1(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
46
2743.9937
(Hex)3(HexNAc)3(NeuAc)2 + (Man)3(GlcNAc)2
18
1870.67
(Hex)2(HexNAc)1(NeuAc)1 + (Man)3(GlcNAc)2
47
2785.025
(Hex)2(HexNAc)4(NeuAc)2 + (Man)3(GlcNAc)2
19
1914.7
(Hex)2(HexNAc)2(dHex)1 + (Man)3(GlcNAc)2
48
2804.0148
(Hex)4(HexNAc)4(NeuAc)1 + (Man)3(GlcNAc)2
20
1955.72
(Hex)1(HexNAc)3(dHex)1 + (Man)3(GlcNAc)2
49
2890.0516
(Hex)3(HexNAc)3(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2
21
1971.72
(Hex)2(HexNAc)3 + (Man)3(GlcNAc)2
50
3049.1048
(Hex)3(HexNAc)3(NeuAc)3 + (Man)3(GlcNAc)2
22
2010.69
(Hex)6 + (Man)3(GlcNAc)2
51
3109.1259
(Hex)4(HexNAc)4(NeuAc)2 + (Man)3(GlcNAc)2
23
2032.72
(Hex)3(HexNac)1(NeuAc)1 + (Man)3(GlcNAc)2
52
3195.1627
(Hex)3(HexNAc)3(dHex)1(NeuAc)3 + (Man)3(GlcNAc)2
24
2057.76
(Hex)1(HexNAc)2(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
53
3354.616
(Hex)3(HexNAc)3(NeuAc)4 + (Man)3(GlcNAc)2
25
2060.76
(Hex)2(HexNAc)2(dHex)2 + (Man)3(GlcNAc)2
54
3414.237
(Hex)4(HexNAc)4(NeuAc)3 + (Man)3(GlcNAc)2
26
2073.75
(Hex)2(HexNAc)2(NeuAc)1 + (Man)3(GlcNAc)2
55
3560.2949
(Hex)4(HexNAc)4(dHex)1(NeuAc)3 + (Man)3(GlcNAc)2
27
2114.78
(Hex)1(HexNAc)3(NeuAc)1 + (Man)3(GlcNAc)2
56
3719.3481
(Hex)4(HexNAc)4(NeuAc)4 + (Man)3(GlcNAc)2
28
2117.78
(Hex)2(HexNAc)3(dHex)1 + (Man)3(GlcNAc)2
57
3865.406
(Hex)4(HexNAc)4(dHex)1(NeuAc)4 + (Man)3(GlcNAc)2
29
2219.81
(Hex)2(HexNAc)2(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2
We identified 56 kinds of -glycan types in the hemodialysis patients. Peak number 35 was an internal standard spiked for quantification. Compositional annotation and putative structures (presented as abbreviations) were collected using the GlycoSuite online database (Proteome Systems). Hex: hexose; HexNAc: -acetyl hexosamine; dHex: deoxyhexose.
