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The Scientific World Journal
Volume 2013, Article ID 275753, 8 pages
Research Article

High Genetic Diversity and Insignificant Interspecific Differentiation in Opisthopappus Shih, an Endangered Cliff Genus Endemic to the Taihang Mountains of China

1Department of Ornamental Horticulture, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin’an, Hangzhou, Zhejiang 311300, China
2Nurturing Station for State Key Laboratory of Subtropical Silviculture, Lin’an, Hangzhou 311300, China
3College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Received 29 September 2013; Accepted 19 November 2013

Academic Editors: A. Belyayev and K. Vandepitte

Copyright © 2013 Rongmin Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Opisthopappus Shih is endemic to the Taihang Mountains, China. It grows in the crevice of cliffs and is in fragmented distribution. This genus consists of two species, namely, O. taihangensis (Ling) Shih and O. longilobus Shih, which are both endangered plants in China. This study adopted intersimple sequence repeat markers (ISSR) to analyze the genetic diversity and genetic structure from different levels (genus, species, and population) in this genus. A total of 253 loci were obtained from 27 primers, 230 of which were polymorphic loci with a proportion of polymorphic bands (PPB) of up to 90.91% at genus level. At species level, both O. taihangensis ( %, , and ) and O. longilobus ( %, , and ) have high genetic diversity. Their respective genetic variation mostly existed within the population. And genetic variation in O. longilobus (84.95%) was higher than that in O. taihangensis (80.45%). A certain genetic differentiation among populations in O. taihangensis was found ( , ) and genetic differentiation in O. longilobus was very small ( , ). Gene flow in different degrees ( and 4.336, resp.) and mating system can form the existing genetic structures of these two species. Furthermore, genetic differentiation coefficient ( ) between species and the clustering result based on the genetic distance showed that interspecific differentiation between O. taihangensis and O. longilobus was not significant and could occur lately.