The Scientific World Journal / 2013 / Article / Tab 4

Research Article

Evaluation of Genotoxic Effects of New Molecules with Possible Trypanocidal Activity for Chagas Disease Treatment

Table 4

PTAL 05-02 induction of mutagenicity and cytotoxicity in Salmonella enterica serovar Typhimurium standard strains, without (−S9) and with (+S9) metabolic activation.

StrainsDose ( g/mL)−S9+S9
Mean ± S.D. (M.I.a)SurvivalbMean ± S.D. (M.I.a)Survivalb

TA97087 ± 9.8 (1.00)10097 ± 25 (1.00)100
0.0589 ± 5.0 (1.03)9067 ± 3.5 (0.70)100
0.189 ± 12 (1.03)10068 ± 4.0 (0.70)92
0.586 ± 8.5 (0.99)10074 ± 0.0 (0.76)90
1.083 ± 1.7 (0.95)10069 ± 4.5 (0.71)80

TA98045 ± 1.5 (1.00)10018 ± 6.4 (1.00)100
0.0530 ± 2.1 (0.67)10018 ± 5.5 (0.96)95
0.138 ± 5.0 (0.83)9821 ± 2.0 (1.16)90
0.538 ± 11 (0.84)10019 ± 1.7 (1.02)81
1.058 ± 11 (1.28)9422 ± 5.2 (1.18)91

TA1000120 ± 23 (1.00)100117 ± 19 (1.00)100
0.05136 ± 15 (1.13)100121 ± 25 (1.04)79
0.1102 ± 25 (0.85)90106 ± 12 (0.91)62
0.584 ± 10 (0.70)100128 ± 18 (1.09)64
1.0105 ± 9.8 (0.88)100129 ± 13 (1.10)49

TA1535022 ± 3.2 (1.00)1005.3 ± 1.5 (1.00)100
0.0521 ± 3.2 (0.94)1005.3 ± 0.5 (1.00)47
0.123 ± 2.0 (1.03)1005.3 ± 0.5 (1.00)42
0.522 ± 4.0 (1.00)976.6 ± 1.5 (1.25)40
1.026 ± 2.0 (1.15)936.3 ± 2.0 (1.19)44

TA1020254 ± 16 (1.00)100265 ± 12 (1.00)100
0.05254 ± 31 (1.00)79253 ± 19 (0.95)100
0.1276 ± 14 (1.09)84225 ± 4.0 (0.85)100
0.5253 ± 11 (1.00)79238 ± 16 (0.90)100
1.0261 ± 14 (1.03)78256 ± 8.3 (0.97)100

Mutagenic index (M.I.): ratio of the his + induced with samples/spontaneous his + in the negative control. S.D.: standard deviation. Positive responses marked in bold when M.I. ≥ 2.
bPercent of cellular survival: <70% was considered toxic dose and it is marked in bold.
*Representation of a significant response ( ).
Negative control was DMSO. Positive controls and their M.I., with and without S9 mix, respectively, were 4NQO (7.19) and 2AA (9.55) for TA97, 4NQO (10) and 2AA (3.95) for TA98; AS (13) and 2AA (2.10) for TA100, AS (3.31) and 2AA (6.25) for TA1535, and MitC (9.28 and 6.86) for TA102.

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