The Scientific World Journal / 2013 / Article / Tab 5

Research Article

Evaluation of Genotoxic Effects of New Molecules with Possible Trypanocidal Activity for Chagas Disease Treatment

Table 5

PAMT 09 induction of mutagenicity and cytotoxicity in Salmonella enterica serovar Typhimurium standard strains, without (−S9) and with (+S9) metabolic activation.

StrainsDose ( g/mL)−S9+S9
Mean ± S.D. (M.I.a)SurvivalbMean ± S.D. (M.I.a)Survivalb

TA97087 ± 9.8 (1.00)10097 ± 25 (1.00)100
0.0589 ± 4.5 (1.02)10074 ± 5.5 (0.76)100
0.187 ± 3.6 (1.02)9584 ± 7.3 (0.87)96
0.592 ± 4.9 (1.07)8587 ± 2.0 (0.90)95
1.081 ± 1.4 (0.93)8380 ± 8.7 (0.82)100

TA98045 ± 1.5 (1.00)10018 ± 6.4 (1.00)100
0.0558 ± 9.6 (1.28)10017 ± 1.0 (0.91)100
0.166 ± 3.0 (1.46)9317 ± 0.5 (0.93)100
0.550 ± 5.8 (1.10)9721 ± 4.0 (1.14)100
1.048 ± 5.8 (1.07)7832 ± 9.0 (1.73)74

TA1000120 ± 23 (1.00)100117 ± 19 (1.00)100
0.05100 ± 18 (0.83)100104 ± 11 (0.89)73
0.196 ± 9.5 (0.80)100126 ± 12 (1.08)73
0.595 ± 9.1 (0.79)100107 ± 2.6 (0.91)72
1.0100 ± 14 (0.83)100107 ± 10 (0.92)71

TA1535022 ± 3.2 (1.00)1005.3 ± 1.5 (1.00)100
0.0519 ± 1.1 (0.94)1007.5 ± 0.7 (1.41)68
0.119 ± 2.3 (0.92)1009.5 ± 2.1 (1.78)55
0.532 ± 5.5 (1.56)1008.6 ± 1.5 (1.63)59
1.025 ± 0.5 (1.21)857.0 ± 1.0 (1.31)58

TA1020254 ± 16 (1.00)100265 ± 12 (1.00)100
0.05285 ± 30 (1.12)79275 ± 17 (1.04)100
0.1289 ± 9.0 (1.14)76242 ± 11 (0.91)100
0.5282 ± 28 (1.11)73256 ± 10 (0.97)100
1.0307 ± 24 (1.21)60240 ± 27 (0.91)100

Mutagenic index (M.I.): ratio of the his + induced with samples/spontaneous his + in the negative control. S.D.: standard deviation. Positive responses marked in bold when M.I. ≥ 2.
bPercent of cellular survival: <70% was considered toxic dose and it is marked in bold.
*Representation of a significant response ( ).
Negative control was DMSO. Positive controls and their M.I., with and without S9 mix, respectively, were: 4NQO (7.19) and 2AA (9.55) for TA97, 4NQO (10) and 2AA (3.95) for TA98; AS (13) and 2AA (2.10) for TA100, AS (18) and 2AA (6.25) for TA1535, and MitC (9.28 and 6.86) for TA102.

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