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The Scientific World Journal
Volume 2013, Article ID 292571, 5 pages
http://dx.doi.org/10.1155/2013/292571
Research Article

Bacillus cereus in Infant Foods: Prevalence Study and Distribution of Enterotoxigenic Virulence Factors in Isfahan Province, Iran

1Department of Food Hygiene and Public Health, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran
2Department of Food Science and Technology, College of Agriculture, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3Department of Microbiology, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
4Infectious Disease and Tropical Medicine Research Center and School of Food Science and Nutrition, Isfahan University of Medical Sciences, Isfahan, Iran

Received 16 March 2013; Accepted 4 May 2013

Academic Editors: S.-J. Ahn and D. Zhou

Copyright © 2013 Ebrahim Rahimi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was carried out in order to investigate the presences of Bacillus cereus and its enterotoxigenic genes in infant foods in Isfahan, Iran. Overall 200 infant foods with various based were collected and immediately transferred to the laboratory. All samples were culture and the genomic DNA was extracted from colonies with typical characters of Bacillus cereus. The presences of enterotoxigenic genes were investigated using the PCR technique. Eighty-four of two hundred samples (42%) were found to be contaminated with B. cereus with a ranges of 3 × 101–9.3 × 101 spore per gram sample. Totally, entFM had the highest (61.90%) incidences of enterotoxigenic genes while hblA had the lowest (13.09%) incidences of enterotoxigenic genes. Overall, 6.7% of B. cereus isolates had all studied enetrotoxigenic genes while 25.5% of B. cereus strains had all studied enetrotoxigenic genes expectance bceT gene. Thisstudyisthe first prevalence report of B. cereus and its enterotoxigenic genes in infant foods in Iran. Results showed that the infant food is one of the main sources of enterotoxigenic genes of B. cereus in Iran. Therefore, the accurate food inspection causes to reducing outbreak of diseases.