The Scientific World Journal / 2013 / Article / Fig 2

Research Article

Association of Self-DNA Mediated TLR9-Related Gene, DNA Methyltransferase, and Cytokeratin Protein Expression Alterations in HT29-Cells to DNA Fragment Length and Methylation Status

Figure 2

Expression fold changes of the assayed genes. (a) Incubation with normally methylated/nonfragmented DNA. TLR9 was overexpressed, and MYD88A and TRAF6 were downregulated. (b) Incubation with normally methylated/fragmented DNA. TLR9, MYD88A, and TRAF6 were overexpressed, and MYD88B, IRF7B, and TNF-alpha were downregulated. (c) Incubation with hypermethylated/nonfragmented DNA. NFκB, IRAK2, and IL8 were overexpressed and TRAF6 was downregulated. (d) Incubation with hypermethylated/fragmented DNA. MYD88A and TRAF6 were overexpressed, and MYD88B, IRAK2, and IL8 and TNF-alpha showed downregulation. 18S was used as a housekeeping gene. Genes with >2 expression fold changes are marked with *; the ones with <0.5-fold change are marked with **.

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