Research Article

Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein

Figure 9

Synchronous fluorescence spectra of interaction between HSA and isoimperatorin (a) at  nm and (b) at  nm. Concentration of HSA was 3.0 μM, while concentrations of isoimperatorin were 0, 0.4, 0.8, 1.2, 1.6, 2, 2.5, and 3 μM from 1 to 8. (c) Comparative evaluation of isoimperatorin effect on the RSFQ of HSA; μM, (■)  nm, and (●)  nm.