Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein

Table 1

Stern-Volmer quenching constant (𝐾_SV), quenching rate constant (𝑘_𝑞), association constant (𝐾_𝑏), and number of binding sites (𝑛) for binding of isoimperatorin to HSA in the different temperatures.

𝑇(K)	10^−4×𝐾SV; (M^−1)	10^−12×𝐾𝑞; (M^−1·s^−1)	𝑅^a	10^−5×𝐾𝑏; (M^−1)	Number of biding sites (𝑛)	𝑅^a
298	16.98±0.13	16.98±0.13	0.9962	11.02±0.10	0.862	0.9841
303	20.19±0.17	20.19±0.17	0.9955	11.49±0.13	0.851	0.9886
308	23.33±0.16	23.33±0.16	0.9965	12.71±0.22	0.844	0.9950
313	26.39±0.19	26.39±0.19	0.9981	13.22±0.17	0.822	0.9917

Data are expressed as mean ± SD of three measurements.
^a𝑅 is the correlation coefficient for the 𝐾_SV values.