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The Scientific World Journal
Volume 2013, Article ID 313482, 6 pages
Research Article

Effects of Chloroform Extract of Dryopteris crassirhizoma on the Ultramicroscopic Structures of Meloidogyne incognita

1School of Life Science, Shanxi University, Taiyuan 030006, China
2Department of Traditional Chinese Medicine, Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, China

Received 28 August 2013; Accepted 23 September 2013

Academic Editors: Y. Moriyasu, N. Suganuma, and S. D. Yeh

Copyright © 2013 Ji-quan Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In our early experiments, the chloroform extract of D. crassirhizoma was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. This study aimed to ascertain the ultrastructural changes in M. incognita after treatment with a D. crassirhizoma chloroform extract at 1 mg·mL−1 for 24 h. It was found that the extract exhibited significant destructive effects on the worm’s ultrastructure and caused distinctive damage to body surfaces and internal structures. These results will contribute to a deeper understanding of the nematicidal mechanism of D. crassirhizoma, as well as in the design of efficient bionematicides.