Improved Tissue Culture Conditions for Engineered Skeletal Muscle Sheets
Figure 2
Multinucleated myofibers in representative muscle tissue sheets (90% C2C12 and 10% Nor-10) cultured in (a) control medium or (b) fCM. Arrows indicate myofibers that stained positive for muscle-specific Desmin (green) and nuclei (blue). Both tissue sheets were fixed at D1 of differentiation.
