Investigation of Biofilm Forming Ability in Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays

Table 1

Primers used in the study, their nucleotide sequence, annealing temperatures, amplicon sizes, and their references.

Gene targeted	Primer sequence 5′-3′; (references)	Annealing temperature °C	Amplicon size bp
16S rRNA	GTA GGT GGC AAG CGTTAT CC; CGC ACA TCA GCG TCA G; Monday and Bohach [19]	55	228
icaA	CCTAACTAACGAAAGGTAG; AAGATATAGCGATAAGTGC; Vasudevan et al. [4]	49	1315
icaD	AAACGTAAGAGAGGTGG; GGCAATATGATCAAGATAC; Vasudevan et al. [4]	49	381
Eno	ACGTGCAGCAGCTGACT; CAACAGCATYCTTCAGTACCTTC; Tristan et al. [20]	55	302
Bap	CCCTATATCGAAGGTGTAGAATTG; GCTGTTGAAGTTAATACTGTACCTGC; Cucarella et al. [21]	60	971