Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α
RP-HPLC analysis of Nα-acetylated Tα1. (a) Sample purified by PoRos R50 column was analyzed by RP-HPLC and eluted as two peaks, I and II corresponding to nonacetylated Tα1 (14.846 min) and Nα-acetylated Tα1 (15.396 min). (b–d) RP-HPLC analysis of samples eluted from SP column with (b) 0.2 M NaCl, (c) 0.3 M NaCl, and (d) 0.5 M NaCl. Nα-acetylated Tα1 was washed out in 0.2 M NaCl fraction (2, peak II), while nonacetylated Tα1 was washed out in 0.5 M NaCl fraction (4, peak I).
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