Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α
Identification of recombinant Nα-terminal acetylated Thymosin α1 by RP-HPLC. Recombinant Nα-acetylated Tα1 was analyzed by RP-HPLC on a C18 column (5 μm, 4.6 × 250 mm) with a programmed acetonitrile gradient in 0.1% TFA, as detailed under “Materials and Methods.” Chromatograms are shown for (a) recombinant Nα-acetylated Tα1 (15.386 min), (b) chemically synthesized Nα-terminal acetylated Tα1 (ZADAXIN, 15.392 min), and (c) mixture of the recombinant Tα1 and chemically synthesized Tα1 (15.392 min).
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