Effect of Vasoactive Intestinal Peptide (VIP) on NKG2D Signal Pathway and Its Contribution to Immune Escape of MKN45 Cells

Table 6

The expression of NKG2D, DAP10, and NF-κB in NK cells incubated with drug.

Group	n	NKG2D protein expression				NKG2D mRNA	DAP10 protein expression				DAP10 mRNA	NF-κB protein expression				NF-κB p65 mRNA
		−	+	++	+++	expression (IOD)	−	+	++	+++	expression (IOD)	−	+	++	+++	expression (IOD)
A	10	0	7	3	0	0.232±0.024	0	0	8	2	0.415±0.009	1	7	2	0	0.201±0.014
B	10	0	1	8	1	0.554±0.031	0	0	3	7	0.617±0.013	0	0	8	2	0.495±0.015
C	10	0	0	4	6	0.742±0.080	0	0	2	8	0.625±0.022	0	0	5	5	0.504±0.027
		𝐻=16.447				𝐹=227.967	𝐻=8.136				𝐹=514.408	𝐻=18.051				𝐹=680.369
		𝑃=0.000				𝑃=0.000	𝑃=0.017				𝑃=0.000	𝑃=0.000				𝑃=0.000

Group A: NK cells + VIP; group B: NK cells + VIP + VIP antagonist; and group C: NK cells (control). IOD: the integrated optical density ratio of the target genes (NKG2D, DAP10, NF-κB) to β-Actin.