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The Scientific World Journal
Volume 2013, Article ID 469674, 9 pages
Research Article

Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.

Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, MD University, Rohtak, Haryana 124001, India

Received 17 August 2013; Accepted 11 September 2013

Academic Editors: A. P. Borole and M. C. Yebra-Biurrun

Copyright © 2013 Kashyap Kumar Dubey and Nitika Goel. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Present work investigated an apposite and efficient method for extraction of betulinic acid (BA) from the bark of Ziziphus jujubae. Various extraction methods like stirring extraction, soxhlet extraction, ultrasonic extraction, and microwave assisted extraction (MAE) were evaluated for increasing recovery percentage of BA. From the raffinate so obtained, BA was isolated. Thin layer chromatography (TLC) was used to analyze the extract and high performance liquid chromatography (HPLC) for quantification. The results revealed that the percentage extraction of BA from Z. jujubae by MAE was more proficient. As recovery percentage of BA by MAE technique turned out to be maximum, by using response surface methodology (RSM), three process parameters (pH, temperature, and time) were optimized by MAE and it was observed that the optimum parameters (pH 6.5, temp. 70.23°C, and time 3.5 min) gave the maximum recovery of BA (0.44% w/w). To validate the RSM model, experiments were performed and the highest recovery of BA was found to be 0.4% w/w which is ±0.04% to the predicted value. Henceforth the extraction efficiency and the substantial saving of time by MAE was more capable than the other extraction techniques.