In Astrocytes the Accumulation of the Immunity-Related GTPases Irga6 and Irgb6 at the Vacuole of Toxoplasma gondii Is Dependent on the Parasite Virulence
Figure 3
Kinetics and localisation of Irga6 and Irgb6 at the PV of avirulent T. gondii. (a) Astrocytes were prestimulated with 100 U/mL IFNγ, pulse-infected with ME49 (MOI: 10) for the indicated time points, and stained for Irga6 and Irgb6. PVs were identified via nucleus staining of tachyzoites with DAPI. Quantitative analysis for both IRGs was performed in three independent experiments at the indicated time points mean ± SEM. Red: Irgb6+ PV, and green: Irga6+ PV, yellow: PV double positive for both analyzed IRGs. ((b), (c)) Astrocytes were prestimulated with IFNγ (100 U/mL), pulse-infected with ME49 for 2 h, stained for Irga6 (green) and Irgb6 (red), and analyzed in a confocal microscope. The experiments were repeated three times with comparable results. (b) Representative image of a parasite in a longitudinal section. (c) Representative layered images through a PV with a distance of 0.29 μm between each layer. Note the different distribution of the two IRGs at the PV while scanning through different layers. Bars: 2 μm.
