Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9

Figure 4

The effects of MMP9 knockdown on the migration of PC3 cells. Phase contrast micrographs of PC3/Sc and PC3/Si cells are shown (a) and (b); (d)–(g). (a)–(c) Phagokinesis assay. The area of the migratory track is seen free of the gold particles. PC3 cells were seen as black spots in the white tracks (a) and (b). Photographs were taken at the same magnification (100× in the lower-power view). The motility was evaluated by measuring areas free of gold particles and represented as mm² (c). Results shown are mean ± SEM of three independent experiments. About 20 to 25 cell tracks from each experiment were measured; ***𝑃<0.001 versus control cells (PC3, PC3/V, and PC3/Sc). (d)–(h) Wound closure assay. A representative wound closure assay before (d) and (e) and after (f) and (g) migration of PC3/Sc (d) and (f) and PC3/Si (e) and (g) is shown. Arrows in (f) and (g) point to dead floating cells. Data showed at the bottom of each panel (in  μm) represent mean ± SEM of an experiment performed in triplicates at 24 h. Statistical analysis (mean ± SEM) of three experiments performed in triplicates at 12 and 24 h is provided as a graph (h). A significant decrease in the migration and wound closure was observed at 12 and 24 h in PC3 cells knockdown of MMP9 (PC3/Si) as compared with indicated control cells. **𝑃<0.01, ***𝑃<0.001 versus control cells (PC3/Sc and PC3/V).