Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome

<table>(a) Eluted phage titers after each round of panning. (b) Western blotting analysis of IPTG-induced Fab antibody expression under nonreducing condition. The protein patterns were visualized using goat anti-human <i><i>λ</i></i> light chain antibodies. (c) Randomly selected clones from 4th panned library were examined for their binding to human Fc fragment by ELISA. GG3 and GG48 denoted previously isolated clones expressing recombinant Fab molecules with Fc-binding activity. BL12 and LPSL are negative controls of irrelevant Fab molecules expressed in <i>E. coli</i>. Bound Fab was detected using goat anti-human <i><i>λ</i></i> light chain antibodies.</table>

The Scientific World Journal

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Figure 1

Figure 1: Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome 

Figure 1 | Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome 