Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 1

Ingredients and nutrients composition of experimental diets.

Ingredient (%)	Starter (1 to 21 d)						Finisher (22 to 42 d)
	M1F0	M2F0	M1F2.5	M2F2.5	M1F5.5	M2F5.5	M1F0	M2F0	M1F2.5	M2F2.5	M1F5.5	M2F5.5
Corn	44.91	44.61	45.61	45.61	45.86	45.61	49.90	49.59	51.45	51.14	51.55	51.14
Soybean meal	43.85	43.60	43.73	43.18	43.48	43.18	38.67	38.47	38.43	38.23	38.33	38.23
Palm oil	6.58	6.58	0.00	0.00	0.00	0.00	7.31	7.31	0.00	0.00	0.00	0.00
Sunflower oil	0.00	0.00	3.50	3.50	0.50	0.50	0.00	0.00	3.50	3.50	0.50	0.50
Tuna oil	0.00	0.00	2.50	2.50	5.50	5.50	0.00	0.00	2.50	2.50	5.50	5.50
Dicalcium phosphate	1.91	1.91	1.91	1.91	1.91	1.91	1.77	1.77	1.77	1.77	1.77	1.77
Limestone	1.20	1.20	1.20	1.20	1.20	1.20	1.06	1.06	1.06	1.06	1.06	1.06
Salt	0.44	0.44	0.44	0.44	0.44	0.44	0.31	0.31	0.31	0.31	0.31	0.31
Vitamin premix^1	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30
Mineral premix^1	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30
DL-Methionine	0.25	0.80	0.25	0.80	0.25	0.80	0.23	0.74	0.23	0.74	0.23	0.74
Lysine	0.26	0.26	0.26	0.26	0.26	0.26	0.15	0.15	0.15	0.15	0.15	0.15
Calculated composition
Crude protein	22.00	22.00	22.00	22.00	22.00	22.00	20.50	20.50	20.50	20.50	20.50	20.50
ME (Kcal/kg)	3080	3080	3080	3080	3080	3080	3150	3150	3150	3150	3150	3150
Available phosphorus	0.45	0.45	0.45	0.45	0.45	0.45	0.42	0.42	0.42	0.42	0.42	0.42
Calcium	1.00	1.00	1.00	1.00	1.00	1.00	0.90	0.90	0.90	0.90	0.90	0.90
Methionine	0.55	1.1	0.55	1.1	0.55	1.1	0.50	1.00	0.50	1.00	0.50	1.00
Lysine	1.20	1.20	1.20	1.20	1.20	1.20	1.00	1.00	1.00	1.00	1.00	1.00
Na	0.20	0.20	0.20	0.20	0.20	0.20	0.15	0.15	0.15	0.15	0.15	0.15
Analyzed composition
Methionine	0.59	1.14	0.63	1.02	0.64	1.06	0.58	0.84	0.61	0.91	0.59	0.90
Lysine	1.26	1.28	1.19	1.29	1.22	1.19	1.11	1.01	1.05	1.13	1.09	1.10
Cysteine	0.42	0.34	0.40	0.38	0.47	0.37	0.35	0.34	0.26	0.35	0.40	0.32
DM	89.60	89.91	89.44	89.80	89.41	89.64	89.95	89.86	90.11	90.45	89.46	89.74
ASH	7.83	5.83	6.67	6.56	6.44	6.53	6.92	5.58	6.96	6.42	6.31	6.25
CP	22.43	21.76	22.45	22.87	21.79	21.85	21.20	21.07	20.40	21.11	21.15	20.88
EE	7.84	7.64	7.70	8.06	7.51	7.11	7.76	8.24	7.01	8.03	7.14	7.78
CF	3.50	3.11	3.64	2.21	3.44	3.68	4.32	4.44	2.95	3.17	3.47	2.50

1Supplied per kilogram of diet: vitamin A: 1,500 IU; cholecalciferol: 200 IU; vitamin E: 10 IU; riboflavin: 3.5 mg; pantothenic acid: 10 mg; niacin: 30 mg; cobalamin: 10 μg; choline chloride: 1,000 mg; biotin: 0.15 mg; folic acid: 0.5 mg; thiamine: 1.5 mg; pyridoxine: 3.0 mg; iron: 80 mg; zinc: 40 mg; manganese, 60 mg; iodine: 0.18 mg; copper: 8 mg; selenium: 0.15 mg; BHT + EQ: 100 mg.
F₀: 0% fish oil; F_2.5: 2.5% fish oil; F_5.5: 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2-fold of NRC).