Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children

Table 4

Amino acid changes in the pre-S region.

Subject	B-cell epitope				T-cell epitope			Region outside the B- and T-cell epitopes
	Pre-S1		Pre-S2		Pre-S1		Pre-S2
	aa 12–32,; aa 27–35,; aa 41–53,; aa 72–78	aa 19–26,; aa 32–47,; aa 37–45,; aa 94–105,; aa 106–117	aa 1–6,; aa 3–15,; aa 13–24	aa 38–48	aa 29–48	aa 12–21,; aa 21–30,; aa 94–105,; aa 106–117	aa 21–30,; aa 29~48
Y1	N48H	Wild-type	G16R	Wild type	N48H	Wild-type	Wild-type	V60A, L85F
Y2	N48H	Wild-type	G16R	Wild type	N48H	Wild-type	Wild-type	M12T, N15S, N20S, T68I, Q82S, L84I, V172A
Y3	N48H	Wild-type	G16R	Wild type	N48H	Wild-type	Wild-type	T68I, Q82S, L84I, V172A
Y4	N48H	Wild-type	G16R	Wild type	N48H	Wild-type	Wild-type	T68I, Q82S, L84I, V172A
Y5	G27D, Q51H, S73G	Wild-type	S6T	Wild type	Wild-type	Wild-type	Wild-type	Q10K, A54E, H56N, V60A, S62A, A90V, L173P

GenBank number D00330 (genotype Ba/serotype adw) was used as the reference sequence for Y1–Y4. GenBank number AF223958 (genotype C/serotype adr) was used as the reference sequence for Y5.