Phenolic Profile of Dark-Grown and Photoperiod-Exposed Hypericum perforatum L. Hairy Root Cultures

Table 1

Retention times, UV, and mass spectral data of phenolic acids, flavonols, and flavan-3-ols in Hypericum perforatum dark-grown (HR1) and photoperiod-exposed (HR2) hairy root culture extracts^a.

Peak no.	Compounds	𝑡𝑅 (min)	UV (nm)	[M–H]^--; (𝑚/𝑧)	–MS^2  [M–H]^--; (𝑚/𝑧)	HR1 (mg⋅100 g^−1 DW ± S.D.)	HR2 (mg⋅100 g^−1 DW ± S.D.)
Phenolic acids
F1	Quinic acid	3.9	262, 310	191	173, 127	166.77±1.20	233.14±19.31
F3	3-p-Coumaroylquinic acid	19.9	314	337	191, 163	n.d.	12.18±0.92
F5	3-Feruloylquinic acid	25.3	314	367	193	n.d.	5.87±0.26
Flavonols
F8	Quercetin 6-C-glucoside	33.9	256, 356	421	331, 301	2.99±0.79	1.74±0.11
F10	Isorhamnetin O-hexoside	38.1	254, 356	477	316, 315, 271	11.80±0.94	1.74±0.09
F11	Kaempferol hexoside	41.2	256, 266, 350	447	285	n.d.	1.99±0.26
F12	Hyperoside (quercetin 3-O-galactoside)	43.8	264, 296 sh, 354	463	301	n.d.	2.77±0.18
F13	Rutin (quercetin 3-O-rutinoside)	44.9	263, 298 sh, 356	609	301	14.72±2.16	5.46±0.43
F14	Quercetin acetylglycoside	48.1	254, 298, 358	505	463, 445, 301	n.d.	3.94±0.10
F15	Kaempferol	59.5	256, 266, 350	285	/	3.92±0.38	6.26±0.37
Flavan-3-ols
F2	Catechin	19.5	280	289	245, 205	27.28±3.20	2.62±0.08
F6	(Epi)catechin	29.9	280	289	245, 205	184.85±12.92	133.36±15.19
F4	Proanthocyanidin dimer	24.5	280	577	559, 451, 425, 407, 289	146.95±9.13	56.61±2.65
F7	Proanthocyanidin dimer	33.4	280	577	559, 451, 425, 407, 289	41.43±1.03	0.76±0.08
F9	Proanthocyanidin dimer	36.8	280	577	559, 451, 425, 407, 289	29.24±2.47	24.93±0.15

an.d.: not detected; DW: dry weight; sh: shoulder; 𝑡_𝑅: retention time. MS² ions in bold indicate the base peak. For information on peak numbers, see Figure 1.