Reestablishment of Ischemia-Reperfusion Liver Injury by N-Acetylcysteine Administration prior to a Preconditioning Iron Protocol
Figure 1
Experimental short-term procedure for N-acetylcysteine (NAC) and/or iron (Fe) administration in the rat (a), ischemia-reperfusion (IR) protocol (b), liver protein carbonyl content (c), liver reduced glutathione (GSH) content (d), and protein carbonyl/GSH content ratios (e). Animals were given either saline or NAC (1 g/kg) or saline followed by Fe (50 mg/kg) or saline, 0.5 h after NAC, at time 0 (controls), 2, 4, 6, 8, and 10 days. Liver oxidative stress-related parameters (protein carbonyl and GSH levels expressed as protein carbonyl/GSH content ratios) were determined at days 11, 12, and 13. At day 13, groups of animals were subjected to sham operation or 1 h ischemia followed by 20 h reperfusion. Blood and liver samples were obtained at the end of the reperfusion period (21 h) for assessment of serum transaminases, liver histology, and hepatic GSH content and p65 NF-B DNA binding. In (c), (d), and (e), data are expressed as means ± SEM for 3 to 11 animals per group. Significance studies (one-way ANOVA and Newman-Keuls’ test; ) are indicated by the letters identifying each experimental group.
