The Scientific World Journal / 2013 / Article / Fig 5

Research Article

Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins

Figure 5

Analysis of GR6 culture filtrates. Panel (a): lane M, SDS-PAGE profile of prestained protein marker (New England Biolabs, USA); lane +ve, purified A. niger β-galactosidase isolated from a culture filtrate of A. niger transformed with the GlaPr-lacA-GlaTt expression cassette; lane 1, culture filtrate prepared using A. oryzae strain GR6 pyrGΔ0; and lane 2, culture filtrate prepared using GR6 pyrGΔ0 transformed with ANEp2. Panel (b); Western blot performed by probing the gel shown in Panel (a) with polyclonal rabbit antibodies directed against A. niger β-galactosidase.